Research and analysis

COVID-19: PHE laboratory assessments of inactivation methods

PHE has undertaken assessments of inactivation methods against SARS-CoV-2 with further evaluations ongoing.

Documents

HCM-CoV2-077-v1_DiaSorin_LIAISON_SARS-CoV-2 Ag_Inactivation_Buffer_TCF

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HCM-CoV2-076-v1 Mologic_COVID_19_Rapid_Antigen_Test _(Swab)_Buffer_TCF

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HCM-CoV2-075-v1_Hypochlorous_Acid_F_K_Holdings_TCF

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HCM-CoV2-074-v1_ScheBo_SARS_CoV_2_Quick_Antigen_Extraction_Buffer_TCF

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HCM-CoV2-073-v1_Zeesan_Saliva_RNA_Sample_Collection_Kit_buffer_TCF

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HCM-CoV2-072-v1_LGC_Salicovgel- TCF

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HCM-CoV2-071-v2_GeneMe_SAVD_Buffer_TCF

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HCM-CoV2-070-v1_GeneMe_FRANKD_Buffer_TCF

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HCM-CoV2-061-v2 Novacyt_PROmate_Sample_Preparation_Buffer _Triton_X_100_(standard)_TCF

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HCM/CoV2/068/v1 MELT V4 Buffer

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HCM/CoV2/067/v1 MELT V3 Buffer

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HCM/CoV2/066/v2 Veritor Extraction Reagent

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HCM/CoV2/065/v1 Salicovgel-1

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HCM/CoV2/064/v1 MicroLYSIS-RNA Direct to PCR Lysis Solution

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HCM/CoV2/063/v1 ID NOW COVID-19 Elution Buffer

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HCM/CoV2/062/v1 taco Sample Storage Solution

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HCM-CoV2-061-v2 Novacyt_PROmate_Sample_Preparation_Buffer _Triton_X_100_(standard)_TCF

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HCM-CoV2-060-v2_Novacyt_PROmate_Sample_Preparation_Buffer_(Triton X-100 reduced)_TCF

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HCM/CoV2/059/v1 virusPHIX-P9

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HCM/CoV2/058/v1 MELT V1

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HCM/CoV2/057/v1 virusPHIX-CU

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HCM/CoV2/056/v1 ESPLINE SARS-CoV-2 Sample Extraction Solution

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HCM-CoV2-055-v1 VirusPHIX-LV

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HCM-CoV2-054-v1 Biomonde Lysis Buffer

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HCM-CoV2-053-v1 Deepblue COVID-19 Antigen Test Extraction Reagent

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HCM-CoV2-052-v1 Orient Gene Coronavirus Ag Rapid Test Buffer

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HCM-CoV2-051-v1 70% Acetone

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HCM-CoV2-050-v1 70% Isopropanol

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HCM-CoV2-049-v1 Abbott Panbio COVID-19 Rapid Test Device Buffer

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HCM-CoV2-048-v1 Innova Extraction Solution

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HCM-CoV2-047-v1 Standard Q COVID-19 Ag Saliva Extraction Buffer

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HCM-CoV2-046-v2 VitaPCR Buffer

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/045/v2 LumiraDx

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HCM-CoV2-044-v1 1% Virkon

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HCM-CoV2-043-v3 Ethanol TCF

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HCM-CoV2-042-v1 Zeesan Biotech Buffer 401108

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HCM-CoV2-041-v1 Zeesan Biotech Buffer 401120

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HCM-CoV2-040-v2 D-Biotech

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HCM-CoV2-039-v2 Omnigene

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HCM/CoV2/038/v4 Standard Q COVID-19 Ag Extraction Buffer

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HCM-CoV2-037-v2 Arcis Buffer

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HCM-CoV2-036-v3 GeneFix Buffer

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HCM-CoV2-035-v3 BuccalFiX Buffer

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HCM-CoV2-034-v2 virusPHIX

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/033/v1 EO Lysis Buffer

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/032/v1 EO VPSS

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-031-v1 PHMB

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-030-v1 Copan eNAT

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-029-v2 MELT Medium B

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-028-v1 MELT Medium A

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-027-v1 Vstrip Extraction Buffer

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-026-v1 Samba SCoV Buffer

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-025-v1 NP40

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-024-v1 SDS

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/023/v1 Triton™ X-100

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/022/v1 Virus Transport and Preservation Medium (Inactivated)

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/021/v1 NeuMoDx™ Viral Lysis Buffer

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-019-v3 4M GITC

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/019/v2 GITC 4M Transport Medium

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/018/v2 AVL + ethanol TCF

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/017/v2 MagNA Pure 96 External Lysis Buffer

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/016/v2 PrimeStore Molecular Transport Medium (MTM)

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/015/v2 cobas® Omni LYS reagent

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/014/v3 GHCl 40% (w/v) Transport Medium

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/013/v3 GITC 2M Transport Medium

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/012/v2 Buffer AVL

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-009-v3 Heat Treatment

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SARS-CoV-2 inactivation testing: interim report HCM/CoV/008/v3 Buffer RLT + 1% β-mercaptoethanol

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/007/v2 Aptima® Specimen Transport Medium

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/006/v3 Triton™ X-100

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/005/v3 Tween® 20 (synonym: polysorbate 20)

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/004/v3 Cobas® PCR Media

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/003/v4 Panther Fusion™ Specimen Lysis Tubes

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/002/v4 Sigma MM™

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SARS-CoV-2 inactivation testing: interim report HCM/CoV/001/v4 Nuclisens® Lysis Buffer

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Details

Public Health England (PHE) has undertaken assessments of inactivation methods against SARS-CoV-2.

Included here are a series of individual interim inactivation reports with further evaluations ongoing.

These reports will be useful for COVID-19 testing laboratories developing workflows that include an inactivation step and will aid the generation of suitable and sufficient risk assessments.

These assessments are now published by UK Health Security Agency.

Published 8 July 2020
Last updated 4 May 2021 + show all updates
  1. Added 11 SARS-CoV-2 inactivation testing interim reports.

  2. Added 12 new reports and updated 043, 050 and 051.

  3. Added updated version of 'HCM-CoV2-043-v3 Ethanol TCF'.

  4. Added reports 34 to 55.

  5. Added 2 SARS-CoV-2 inactivation testing interim reports.

  6. Added 12 SARS-CoV-2 inactivation testing interim reports.

  7. First published.