Research and analysis

COVID-19: PHE laboratory assessments of inactivation methods

PHE has undertaken assessments of inactivation methods against SARS-CoV-2 with further evaluations ongoing.

From:
Public Health England
Published
8 July 2020
Last updated
4 May 2021 — See all updates

Documents

HCM-CoV2-077-v1_DiaSorin_LIAISON_SARS-CoV-2 Ag_Inactivation_Buffer_TCF

Ref: GW-8122 PDF, 149 KB, 5 pages

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HCM-CoV2-076-v1 Mologic_COVID_19_Rapid_Antigen_Test _(Swab)_Buffer_TCF

Ref: GW-8122 PDF, 118 KB, 4 pages

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HCM-CoV2-075-v1_Hypochlorous_Acid_F_K_Holdings_TCF

Ref: GW-8122 PDF, 133 KB, 4 pages

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HCM-CoV2-074-v1_ScheBo_SARS_CoV_2_Quick_Antigen_Extraction_Buffer_TCF

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HCM-CoV2-073-v1_Zeesan_Saliva_RNA_Sample_Collection_Kit_buffer_TCF

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HCM-CoV2-072-v1_LGC_Salicovgel- TCF

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HCM-CoV2-071-v2_GeneMe_SAVD_Buffer_TCF

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HCM-CoV2-070-v1_GeneMe_FRANKD_Buffer_TCF

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HCM-CoV2-061-v2 Novacyt_PROmate_Sample_Preparation_Buffer _Triton_X_100_(standard)_TCF

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HCM/CoV2/068/v1 MELT V4 Buffer

Ref: PHE publications gateway number GW-1859 PDF, 212 KB, 4 pages

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HCM/CoV2/067/v1 MELT V3 Buffer

Ref: PHE publications gateway number GW-1859 PDF, 212 KB, 4 pages

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HCM/CoV2/066/v2 Veritor Extraction Reagent

Ref: PHE publications gateway number GW-1859 PDF, 216 KB, 4 pages

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HCM/CoV2/065/v1 Salicovgel-1

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HCM/CoV2/064/v1 MicroLYSIS-RNA Direct to PCR Lysis Solution

Ref: PHE publications gateway number GW-1859 PDF, 213 KB, 4 pages

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HCM/CoV2/063/v1 ID NOW COVID-19 Elution Buffer

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HCM/CoV2/062/v1 taco Sample Storage Solution

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HCM-CoV2-061-v2 Novacyt_PROmate_Sample_Preparation_Buffer _Triton_X_100_(standard)_TCF

Ref: GW-8122 PDF, 115 KB, 4 pages

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HCM-CoV2-060-v2_Novacyt_PROmate_Sample_Preparation_Buffer_(Triton X-100 reduced)_TCF

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HCM/CoV2/059/v1 virusPHIX-P9

Ref: PHE publications gateway number GW-1859 PDF, 214 KB, 4 pages

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HCM/CoV2/058/v1 MELT V1

Ref: PHE publications gateway number GW-1859 PDF, 213 KB, 4 pages

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HCM/CoV2/057/v1 virusPHIX-CU

Ref: PHE publications gateway number GW-1859 PDF, 212 KB, 4 pages

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HCM/CoV2/056/v1 ESPLINE SARS-CoV-2 Sample Extraction Solution

Ref: PHE publications gateway number GW-1859 PDF, 214 KB, 4 pages

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HCM-CoV2-055-v1 VirusPHIX-LV

Ref: PHE publications gateway number GW-1725 PDF, 353 KB, 4 pages

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HCM-CoV2-054-v1 Biomonde Lysis Buffer

Ref: PHE publications gateway number GW-1725 PDF, 361 KB, 4 pages

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HCM-CoV2-053-v1 Deepblue COVID-19 Antigen Test Extraction Reagent

Ref: PHE publications gateway number GW-1725 PDF, 352 KB, 4 pages

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HCM-CoV2-052-v1 Orient Gene Coronavirus Ag Rapid Test Buffer

Ref: PHE publications gateway number GW-1725 PDF, 353 KB, 4 pages

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HCM-CoV2-051-v1 70% Acetone

Ref: PHE publications gateway number GW-1859 PDF, 242 KB, 5 pages

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HCM-CoV2-050-v1 70% Isopropanol

Ref: PHE publications gateway number GW-1859 PDF, 228 KB, 4 pages

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HCM-CoV2-049-v1 Abbott Panbio COVID-19 Rapid Test Device Buffer

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HCM-CoV2-048-v1 Innova Extraction Solution

Ref: PHE publications gateway number GW-1725 PDF, 353 KB, 4 pages

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HCM-CoV2-047-v1 Standard Q COVID-19 Ag Saliva Extraction Buffer

Ref: PHE publications gateway number GW-1725 PDF, 353 KB, 4 pages

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HCM-CoV2-046-v2 VitaPCR Buffer

Ref: PHE publications gateway number GW-1725 PDF, 371 KB, 5 pages

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/045/v2 LumiraDx

Ref: PHE publications gateway number: GW-1694 PDF, 186 KB, 4 pages

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HCM-CoV2-044-v1 1% Virkon

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HCM-CoV2-043-v3 Ethanol TCF

Ref: PHE publications gateway number GW-1859 PDF, 243 KB, 5 pages

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HCM-CoV2-042-v1 Zeesan Biotech Buffer 401108

Ref: PHE publications gateway number GW-1725 PDF, 356 KB, 4 pages

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HCM-CoV2-041-v1 Zeesan Biotech Buffer 401120

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HCM-CoV2-040-v2 D-Biotech

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HCM-CoV2-039-v2 Omnigene

Ref: PHE publications gateway number GW-1725 PDF, 356 KB, 4 pages

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HCM/CoV2/038/v4 Standard Q COVID-19 Ag Extraction Buffer

Ref: PHE publications GW-1859 PDF, 219 KB, 4 pages

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HCM-CoV2-037-v2 Arcis Buffer

Ref: PHE publications gateway number GW-1725 PDF, 381 KB, 5 pages

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HCM-CoV2-036-v3 GeneFix Buffer

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HCM-CoV2-035-v3 BuccalFiX Buffer

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HCM-CoV2-034-v2 virusPHIX

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/033/v1 EO Lysis Buffer

Ref: PHE publications gateway number: GW-1464 PDF, 95.1 KB, 4 pages

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/032/v1 EO VPSS

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-031-v1 PHMB

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-030-v1 Copan eNAT

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-029-v2 MELT Medium B

Ref: PHE publications gateway number: GW-1464 PDF, 189 KB, 4 pages

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-028-v1 MELT Medium A

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-027-v1 Vstrip Extraction Buffer

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-026-v1 Samba SCoV Buffer

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-025-v1 NP40

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-024-v1 SDS

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/023/v1 Triton™ X-100

Ref: PHE publications gateway number GW-1373 PDF, 324 KB, 4 pages

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/022/v1 Virus Transport and Preservation Medium (Inactivated)

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/021/v1 NeuMoDx™ Viral Lysis Buffer

Ref: PHE publications gateway number GW-1373 PDF, 323 KB, 4 pages

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-019-v3 4M GITC

Ref: PHE publications gateway number: GW-1464 PDF, 211 KB, 5 pages

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/019/v2 GITC 4M Transport Medium

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/018/v2 AVL + ethanol TCF

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/017/v2 MagNA Pure 96 External Lysis Buffer

Ref: PHE publications gateway number GW-1373 PDF, 323 KB, 4 pages

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/016/v2 PrimeStore Molecular Transport Medium (MTM)

Ref: PHE publications gateway number GW-1373 PDF, 325 KB, 4 pages

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/015/v2 cobas® Omni LYS reagent

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/014/v3 GHCl 40% (w/v) Transport Medium

Ref: PHE publications gateway number GW-137 PDF, 331 KB, 5 pages

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/013/v3 GITC 2M Transport Medium

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/012/v2 Buffer AVL

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SARS-CoV-2 inactivation testing: interim report HCM-CoV2-009-v3 Heat Treatment

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SARS-CoV-2 inactivation testing: interim report HCM/CoV/008/v3 Buffer RLT + 1% β-mercaptoethanol

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/007/v2 Aptima® Specimen Transport Medium

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/006/v3 Triton™ X-100

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/005/v3 Tween® 20 (synonym: polysorbate 20)

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/004/v3 Cobas® PCR Media

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/003/v4 Panther Fusion™ Specimen Lysis Tubes

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SARS-CoV-2 inactivation testing: interim report HCM/CoV2/002/v4 Sigma MM™

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SARS-CoV-2 inactivation testing: interim report HCM/CoV/001/v4 Nuclisens® Lysis Buffer

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Details

Public Health England (PHE) has undertaken assessments of inactivation methods against SARS-CoV-2.

Included here are a series of individual interim inactivation reports with further evaluations ongoing.

These reports will be useful for COVID-19 testing laboratories developing workflows that include an inactivation step and will aid the generation of suitable and sufficient risk assessments.

These assessments are now published by UK Health Security Agency.

Published 8 July 2020
Last updated 4 May 2021 + show all updates

  1. Added 11 SARS-CoV-2 inactivation testing interim reports.

  2. Added 12 new reports and updated 043, 050 and 051.

  3. Added updated version of 'HCM-CoV2-043-v3 Ethanol TCF'.

  4. Added reports 34 to 55.

  5. Added 2 SARS-CoV-2 inactivation testing interim reports.

  6. Added 12 SARS-CoV-2 inactivation testing interim reports.

  7. First published.

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