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Biological reference points (BRPs) provide a means of assessing the status of fish stocks against a defined reference condition. These assessments help to identify and prioritise management actions to ensure that fish populations reach a favourable status. The Environment Agency uses BRPs to assess salmon populations in rivers in England and Wales. However, a BRP method has not been applied to any other freshwater fish species in England and Wales, largely due to the dearth of speciesspecific data and information such as lifecycle variation.