Junction 12 at Gaydon will be fully closed from 8pm on Friday 24 June to 5am to Monday 27 June to enable road widening and resurfacing work.
Work will also be taking place on both the northbound and southbound entry and exit slip roads.
Highways England project manager, Shah Hussain said:
We’re working over the weekend to minimise disruption to motorists and to make sure that we can use the closure to make good progress on the work.
A diversion route will be signposted for the duration of the work.
Junction 12 is extremely busy, with queues of up to 1.2 miles forming on the M40 southbound hard shoulder during the morning peak. The new scheme, near Gaydon, aims to cut queuing, boost safety and improve journeys, while unlocking potential economic growth in the area.
The Highways England scheme, in conjunction with the work to create a dual carriageway on the B4451 being undertaken currently by Warwickshire County Council, will greatly add to the capacity of local roads and help support the growing local economy.
Members of the public should contact the Highways England customer contact centre on 0300 123 5000.
Journalists should contact the Highways England press office on 0844 693 1448 and use the menu to speak to the most appropriate press officer.