The eastbound carriageway of Humber Road between Habrough Road Roundabout and Eastfield Road, South Killingholme, will be closed overnight between 8pm to 6am on Wednesday 13 May.
The westbound carriageway will be closed overnight between 8pm to 6am on Thursday 14 May.
Following each closure narrow lanes and a reduced 40mph speed limit will be put in place.
Diverted eastbound traffic for the Port of Immingham will stay on A180 leaving at Stallingborough roundabout. From there drivers will follow the A1173 Kings Road before joining the A1173 Manby road to the Port of Immingham.
Westbound traffic leaving the Port of Immingham will follow the A1173 Manby Road, the A1173 Kings Road, before re- joining the A180 at Stallingborough.
The new bridge will provide vehicle and pedestrian access between the two areas of South Killingholme.
The works are part of an improvement programme designed to provide better access to the port and the surrounding area.
Members of the public should contact the Highways England customer contact centre on 0300 123 5000.
Journalists should contact the Highways England press office on 0844 693 1448 and use the menu to speak to the most appropriate press officer.