Transformation of sweet potato (Ipomoea batatas (L) Lam) with Agrobacterium tumefaciens and regeneration of plants expressing cowpea trypsin inhibitor and snowdrop lectin
Abstract
Transgenic sweet potato plants were obtained following transformation with Agrobacterium tumefaciens LBA4404. Discs cut from freshly-harvested storage roots of the sweet potato cultivar ‘Jewel’, were inoculated and cultured on various media to induce regeneration. Regeneration occurred via somatic embryogenesis, but somatic embryos were only successfully induced to germinate and produce shoots after removal of antibiotics from the tissue culture medium. Kanamycin monosulphate was used in the initial stages of culture as a selective agent for transformed tissue. Plants were confirmed as transgenic by Southern analysis of DNA, RNA dot blot analysis, and protein analysis using enhanced chemiluminescence detection methods. Transgenic plants have been obtained that express the β-glucuronidase enzyme, the cowpea trypsin inhibitor and the snowdrop lectin.
Citation
Newell, C.A.; Lowe, J.M.; Merryweather, A.; Rooke, L.M.; Hamilton, W.D.O. Transformation of sweet potato (Ipomoea batatas (L.) Lam.) with Agrobacterium tumefaciens and regeneration of plants expressing cowpea trypsin inhibitor and snowdrop lectin. Plant Science (1995) 107 (2) 215-227. [DOI: 10.1016/0168-9452(95)04109-8]