Independent report

Vitamin B12: A review of analytical methods for use in food

A summary of analytical methods available for the determination of vitamin B12 in fortified foods or supplements.

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This technical report outlines the role of vitamin B12 in the diet and explains why groups such as infants, the elderly, and vegetarians or vegans can be prone to deficiency.

The report summarises analytical methods available for the determination of vitamin B12 in food to enable confirmation of label declarations. Methods include microbiological assay, polarographic, spectrophotometric, radio-ligand binding and various chromatographic techniques.