The work, which is part of a comprehensive package of bridge safety improvements and maintenance to benefit road users on the M5 near Gloucester, will take place between 10pm and 6am.
An overnight closure of the B4073 underneath the bridge between Corncroft Lane and Birchall Lane will also be needed to ensure the safety of road users. Clearly signed diversions will be in place.
Commenting on the works, Project Sponsor Dave Frampton said:
The closure of lanes one and two northbound represents Phase Three of this work, which involves the removal of the bridge beams. The whole scheme is proceeding well and we are on schedule to complete the works on time.
Full details and regular updates on the works, including videos and photos, can be found on our dedicated M5 Gloucestershire Bridge Works Facebook page here.
Members of the public should contact the Highways Agency Information Line on 0300 123 5000.
Journalists should contact the Highways Agency press office on 0844 693 1448 and use the menu to speak to the most appropriate press officer.