Viral Inhibition Assay: A CD8 T Cell Neutralization Assay for Use in Clinical Trials of HIV-1 Vaccine Candidates

From:
Department for International Development
Published
1 January 2010
Document Type:
Journal Article
Theme:
Health
Authors:
Wong, J., Gotch, F., Ashraf, A., Karita, E., Anzala, O., Dally, L., Gilmour, J., Hayes, P., Walker, B., Anjarwalla, I., Bergin, P., Cashin-Cox, M., Cheeseman, H., Gill, D., Higgs, C., Kaltsidis, H., Piechocka-Trocha, A., Pozniak, A., Spentzou, A., Steel, A.

Abstract

We have characterized an assay measuring CD8 T cell-mediated inhibition of human immunodeficiency virus (HIV) type 1 replication, demonstrating specificity and reproducibility and employing a panel of primary HIV-1 isolates. The assay uses relatively simple autologous cell culture and enzyme-linked immunosorbent assay, avoids generation of T cell clones, and can be performed with

Citation

Spentzou, A., Bergin, P., Gill, D., Cheeseman, H., Ashraf, A., Kaltsidis, H., Cashin-Cox, M., Anjarwalla, I., Steel, A., Higgs, C., Pozniak, A., Piechocka-Trocha, A., Wong, J., Anzala, O., Karita, E., Dally, L., Gotch, F, Walker, B., Gilmour, J., Hayes, P. Viral Inhibition Assay: A CD8 T Cell Neutralization Assay for Use in Clinical Trials of HIV-1 Vaccine Candidates. Journal of Infectious Diseases (2010) 201 (5) 720-729. [DOI: 10.1086/650492]

Viral Inhibition Assay: A CD8 T Cell Neutralization Assay for Use in Clinical Trials of HIV-1 Vaccine Candidates

Published 1 January 2010
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