Validation and clinical evaluation of a novel method to measure miltefosine in leishmaniasis patients using dried blood spot sample collection

This research was conducted in collaboration with the Drugs for Neglected Diseases Initiative

Abstract

To facilitate future pharmacokinetic studies of combination treatments against leishmaniasis in remote endemic regions, a simple and cheap sampling methodology was required for miltefosine quantification.

The aim of this study was to validate a liquid chromatography-tandem mass spectrometry method to quantify miltefosine in dried blood spots, and to validate its use in Ethiopian visceral leishmaniasis patients.  The method was demonstrated to be a valid and practical alternative to testing blood sampled from veins, and can be applied in future miltefosine pharmacokinetic studies in leishmaniasis patients.

This research was conducted in collaboration with the Drugs for Neglected Diseases Initiative (DNDi)

Citation

Kip AE, Rosing H, Hillebrand MJX, Blesson S, Mengesha B, Diro E, Hailu A, Schellens JHM, Beijnen JH, Dorlo TPC. 2016. Validation and clinical evaluation of a novel method to measure miltefosine in leishmaniasis patients using dried blood spot sample collection. Antimicrob Agents Chemother 60:2081–2089. doi:10.1128/AAC.02976-15.

Validation and clinical evaluation of a novel method to measure miltefosine in leishmaniasis patients using dried blood spot sample collection

Published 1 January 2016