The objectives of the project were to investigate the major factors inhibiting the cryopreservation of fish eggs and embryos.
This report provides an executive summary of the work conducted followed by details of the results and findings of studies on invertebrates (cryopreservation of oyster eggs and embryos, and Artemia salina embryos), fish eggs and embryos (low temperature tolerance, membrane permeability, measurement of osmotically inactive water, development of in vitro techniques for eggs of new model species, procurement of gametes and embryos, dechorionation of eggs and embryos, determination of the permeability of egg membranes to water, effect of electroporation on the viability and permeability of eggs and embryos, prefreezing toxicity of cryoprotectants to fish embryos, and short term preservation of eggs) and spermatozoa (assessment of cryopreservation protocols for milt, cooling rates and development of a multichannel temperature data logger, urine contamination of milt, cryopreservation of carp milt, storage of UV-irradiated milt, cryopreservation of milt extracted from testis, enhancement of the duration of sperm motility, and preliminary studies on the activation of immotile spermatozoa). The implications of the results, and priority tasks and future directions are discussed, and a summary of financial expenditure is included.
The cryopreservation and manipulation of ova and milt in commercially important tropical species. Final Project Report. Institute of Aquaculture, Stirling, UK, 81 pp.