The five fish species were challenged with Aphanomyces invadans, the fungus considered to be the causative agent of epizootic ulcerative syndrome (EUS). At least 16 fish of each species were injected intramuscularly with A invadans, kept at 11-24 C depending of species, and periodically sampled for histology and immunohistochemistry. The potential for transmission of EUS to some temperate fish species (such as rainbow trout and roach) via susceptible ornamental species like rosy barbs is demonstrated.
Khan, M.H.; Marshall, L.; Thompson, K.D.; Campbell, R.E.; Lilley, J.H. Susceptibility of five fish species (Nile tilapia, rosy barb, rainbow trout, stickleback and roach) to intramuscular injection with the Oomycete fish pathogen, Aphanomyces invadans. European Association of Fish Pathologists Bulletin (1998) 18 (6) 192-197.