Recombinant microsatellite amplification: a rapid method for developing simple sequence repeat markers

Abstract

Recombinant microsatellite (simple sequence repeat; SSR) amplification is a technique by which DNA sequences flanking microsatellites can be isolated rapidly on a large scale. The approach selectively amplifies microsatellite-containing sequences and recombines the amplicons by redigestion and ligation, in order to increase the yield of microsatellite flanks per clone two-fold and to further increase selectivity of amplification. Since this method does not require prior knowledge of the genomic sequence, it is especially useful for species for which abundant genomic sequences are not available. The feasibility of this approach was demonstrated by developing SSR markers in cultivated oats.

Citation

Bin Wu; Ping Lu; Zhang ZongWen. Recombinant microsatellite amplification: a rapid method for developing simple sequence repeat markers. Molecular Breeding (2012) 29 (1) 53-59. [DOI: 10.1007/s11032-010-9525-y]

Recombinant microsatellite amplification: a rapid method for developing simple sequence repeat markers

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