QTLs meet genomics: targeting superior disease QTL alleles in the rice genome.
Extensive genetic mapping data coupled with the complete sequence of the rice genome has enabled us to combine classical genetics and comparative genomics to target superior disease quantitative trait loci (dQTLs) associated with resistance to multiple diseases in cereals. We have previously identified and mapped candidate genes (CGs) conferring quantitative resistance to rice blast concomitant with selection for good agronomic traits in an advanced backcross populations of Vandana x Moroberekan. Using gene-based primers as well as simple sequence repeat markers, we identified oxalate oxidase, oxalate oxidase-like protein, thaumatin, and peroxidase significantly correlated with blast resistance in 108 introgression lines (84 selected for blast resistance and 24 for drought tolerance). We have also characterized the chromosomal regions containing dQTLs by in silico and expression approaches. Information from this study as well as gene-based molecular markers developed were used to identify candidate genes associated with resistance to rice blast in an advance backcross population of Way Rarem (WR) x Oryzica Llanos 5 (OL5).
Carrillo, G.; Ona, I.; Lestari, P.; Qui, F.; Goodwin, P.; Variar, M.; Bustamam, M.; Leach, J.; Nelson, R.; Leung, H.; Vera Cruz, C. QTLs meet genomics: targeting superior disease QTL alleles in the rice genome. Presented at the 2006 GCP-Annual Research Meeting, 12-16 September 2006, Sao Paolo, Brazil. (2006) 1 pp.