Poly(methacrylic acid) complexation of amphotericin B to treat neglected diseases

Abstract

Amphotericin B (AmB) is used to treat a neglected disease called visceral leishmaniasis (VL). We hypothesised that direct non-covalent association of AmB with poly(methacrylic acid) (PMAA) would replicate many of the properties of liposomal AmB (AmB-L), which is more efficacious than micellar AmB (AmB-D). Water-soluble AmB–PMAA complexes with AmB loadings ranging from 20 to 45% were reproducibly prepared. The AmB in the PMAA complex displayed similar aggregation properties to the AmB within AmB-L. The AmB–PMAA complex displayed low heamolytic properties while maintaining in vitro activity against Leishmania donovani amastigotes with no macrophage toxicity observed at an IC50 of 0.043 (±0.003) μM. AmB–PMAA complexes were well tolerated in vivo at a total dose of 6 mg kg−1 and both the complex (2.2 mg kg−1 AmB) and AmB-L (2.5 mg kg−1 AmB) achieved greater than 90% parasite inhibition in vivo after a single dose against L. donovani in HU3 infected BALB/c mice.

Citation

Les, K.A.; Mohamed-Ahmed, A.H.A.; Balan, S.; Choi JiWon; Martin, D.; Yardley, V.; Powell, K.; Godwin, A.; Brocchini, S. Poly(methacrylic acid) complexation of amphotericin B to treat neglected diseases. Polymer Chemistry (2014) 5 (3) 1037-1048. [DOI: 10.1039/c3py01051a]

Poly(methacrylic acid) complexation of amphotericin B to treat neglected diseases

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