A flow cytometry antibody panel was developed and optimized for the characterization of CD4+ and CD8+ cell memory and functional responses in adult and infant cryopreserved peripheral blood mononuclear cells (PBMC) stimulated with peptide pools to various antigens of interest.The panel has been used to evaluate Mycobacterium tuberculosis(TB) antigen-specific responses in clinical trial specimens, and is currently undergoing assay qualification.
Graves, A.J.; Padilla, M.G.; Hokey, D.A. OMIP-022: Comprehensive assessment of antigen-specific human T-cell functionality and memory. Cytometry Part A (2014) 85 (7) 576-579. [DOI: 10.1002/cyto.a.22478]