OMIP-022: Comprehensive Assessment of Antigen-Specific Human T-Cell Functionality and Memory

A flow cytometry antibody panel was developed and optimised

From:
Department for International Development
Published
1 January 2014
Document Type:
Journal Article
Theme:
Health
Authors:
Hokey, D.A., Graves, A.J., Padilla, M.G.

Abstract

A flow cytometry antibody panel was developed and optimized for the characterization of CD4+ and CD8+ cell memory and functional responses in adult and infant cryopreserved peripheral blood mononuclear cells (PBMC) stimulated with peptide pools to various antigens of interest.The panel has been used to evaluate Mycobacterium tuberculosis(TB) antigen-specific responses in clinical trial specimens, and is currently undergoing assay qualification.

Citation

Graves, A.J.; Padilla, M.G.; Hokey, D.A. OMIP-022: Comprehensive assessment of antigen-specific human T-cell functionality and memory. Cytometry Part A (2014) 85 (7) 576-579. [DOI: 10.1002/cyto.a.22478]

OMIP-022: Comprehensive Assessment of Antigen-Specific Human T-Cell Functionality and Memory

Published 1 January 2014
Contents