Molecular diversity amongst populations of the burrowing nematode, Radopholus similis, occurring in Sri Lanka
DNA extracted from fourteen populations of Radopholus similis, collected previously from roots of various hosts at different locations in Sri Lanka, and one new species of Radopholus from East Java was compared using the polymerase chain reaction based method of random amplified polymorphie DNA (RAPD). Four populations isolated from arecanut, tea, banana and citrus appeared to be particularly different in their RAPD profiles. Hierarchical cluster analysis was applied to the data obtained from RAPD patterns. This arranged most of the fifteen populations into three putative groups and revealed three more divergent isolates which are, thus far, monotypic. The results are discussed in the context of the genetic divergence and the existence of biotypes of R. similis in Sri Lanka.
Bridge, J.; Burrows, P.R.; Gnanapragasam, N.C.; Hahn, M.L.; Vines, N.J.; Wright, D.J. Molecular diversity amongst populations of the burrowing nematode, Radopholus similis, occurring in Sri Lanka. Fundamental and applied nematology (1994) 17 (3) 275-281.