Multiple vegetative shoots and flowers emerged from the nodes of explants derived from young seedlings of the bamboo Dendrocalamus hamiltonii when cultured on a Murashige & Skoog based medium supplemented with the cytokinin 6-benzyladenine (BA). The largest number of shoots produced from intact cultured epicotyl tissue (ca 8 per explant) were obtained after 12 weeks on a medium containing 4.4 M BA. The first flowers were observed in vitro after only 13 weeks from initial germination of the seedlings. The highest frequency of flowering (47% of nodal explants) was obtained when cultured explants were transferred from 22.2 M BA to a growth regulator-free medium after 8 weeks.
Plant Cell, Tissue and Organ Culture (1991) 27 (1) 45-48 [10.1007/BF00048205]
Micropropagation and in vitro flowering of the bamboo Dendrocalamus hamiltonii Munro