Micropropagation and in vitro flowering of the bamboo Dendrocalamus hamiltonii Munro
Multiple vegetative shoots and flowers emerged from the nodes of explants derived from young seedlings of the bamboo Dendrocalamus hamiltonii when cultured on a Murashige & Skoog based medium supplemented with the cytokinin 6-benzyladenine (BA). The largest number of shoots produced from intact cultured epicotyl tissue (ca 8 per explant) were obtained after 12 weeks on a medium containing 4.4 M BA. The first flowers were observed in vitro after only 13 weeks from initial germination of the seedlings. The highest frequency of flowering (47% of nodal explants) was obtained when cultured explants were transferred from 22.2 M BA to a growth regulator-free medium after 8 weeks.
Plant Cell, Tissue and Organ Culture (1991) 27 (1) 45-48 [10.1007/BF00048205]