AFLP marker data were generated for a doubled haploid rice mapping population developed from a cross between IR64 (indica) and Azucena (japonica) varieties, as well as for a diverse set of rice germplasm. A total of 151 AFLP markers were incorporated into an existing restriction fragment length polymorphism framework map and compared with a published map. Markers associated with the indica–japonica subspecific differentiation in Asian rice were also mapped and were found to be distributed throughout the genome. Sources of mapping errors were examined and the possibility of such errors being caused by co-migration of bands and segregation distortion was explored. In the case of markers that show distorted segregation, it was concluded that one-third of these may be affected by gametophytic/sterility genes and a futher one-third may arise because of association with the indica–japonica differentiation.
Virk, P.S.; Ford-Lloyd, B.V.; Newbury, H.J. Mapping AFLP markers associated with subspecific differentiation of Oryza sativa (rice) and an investigation of segregation distortion. Heredity (1998) 81 (6) 613-620. [DOI: 10.1046/j.1365-2540.1998.00441.x]