Low cost, non gel-based DNA marker technologies for marker assisted selection in rice.
Currently, two obstacles for marker assisted selection (MAS) are the high cost for genotyping and limited sample throughput capacity. We are developing new alternative genotyping methods in a collaborative project between IRRI, CIMMYT and NARES partner institutions. Two methods are being developed as low cost approaches that can be adopted in laboratories with limited resources. Two other methods are being developed as high-throughput and cost-effective methods that could be utilized in core genotyping institute. We are validating these methods using previously developed markers, characterized or cloned bacterial blight resistance genes (xa5, Xa7, and Xa21) in rice.
Reveche, M.Y.; Mercado, E.; Chen, J.; Carrillo, G.; Bernardo, M.; Wu, J.; Redoña, E.; Kadirvel, P.; Bustamam, M.; Verdier, V.; William, M.; Vera Cruz, C.M.; Collard, B.C.Y. Low cost, non gel-based DNA marker technologies for marker assisted selection in rice. (2005) 1 pp.