Longitudinal assessment of HIV-1 and HSV-2 shedding in the genital tract of West African women.

From:
Department for International Development
Published
1 January 2005
Country:
Burkina Faso
Document Type:
Journal Article
Theme:
Health
Authors:
Mayaud, P., Weiss, H., Nagot, N., Ouedraogo, A., Defer, M.C., van de Perre, P., Segondy, M., Konate, I., Foulongne, V., Becquart, P.

Abstract

Forty-two HSV-2-seropositive women, of whom 22 were HIV-1 seropositive, were followed up weekly for 3 weeks for assessment of HIV-1 and HSV-2 genital shedding in Bobo Dioulasso, Burkina Faso. HSV-2 DNA and HIV-1 RNA were quantitated in cervicovaginal lavages enriched with a cervical swabbing (eCVL) using real-time polymerase chain reaction. HIV-1 RNA shedding was detected in eCVL from 19 of 22 (86.4%) of the HIV-1-seropositive women and HSV-2 DNA was detected in eCVL from 12 of 42 (28.6%) of the women. Compared with the HIV-1-seronegative women, the HIV-1-seropositive women showed more persistent HSV-2 genital shedding and higher HSV-2 DNA loads in eCVL. This study showed that eCVL is a reliable sampling method for detecting both viruses. Three measurement points (at weekly intervals in this study) seem to adequately allow detection of most HIV-1 or HSV-2 genital shedders.

Citation

Journal of Acquired Immune Deficiency Syndromes (2005) 39 (5) pp. 632-634.

Published 1 January 2005
Contents