Differential Display RT-PCR was developed before the genomic era to serve as a tool in hunting for genes. Nowadays, applications using state-of-the-art techniques to obtain more information about the whole transcriptome or the genome have rapidly overtaken DD-RT-PCR. This paper will discuss a few of the major drawbacks and limitations of using this once highly valued method.
Soós, V.; Juhász, A.; Sebestyén, E.; Light, M.E.; Staden, J.; Balázs, E. Limitations of using Differential Display RT-PCR in the chase for smoke-related genes. Acta Agronomica Hungarica (2008) 56 (4) 435-441. [DOI: 10.1556/AAgr.56.2008.4.9]