The project aimed to investigate the fundamental constraints associated with the cryostorage of fish eggs and embryos. This report provides an executive summary, details of the project objectives, a description of the work carried out and the results obtained, and discusses the implications of the results.
Techniques for maintaining, breeding and procuring gametes were successfully developed for 2 selected model species, the zebra fish (Brachydanio rerio) and the rosy barb (Puntium conchonius). Results of studies on the precooling toxicity of cryoprotectants to embryos, the effect of temperature and chorion manipulation on survival and permeability of embryos, estimation of osmotically active and non-active water fractions in embryos, the effects of cold shock and subzero cooling on embryo survival, the effects of cooling rate and subzero temperature on survival of isolated blastomeres and vitrification of heartbeat stage embryos are reported.
Investigations into the problems of cryostorage of eggs and embryos of commercially important tropical species. Final Project Report, University of Stirling, UK, 83 pp.