Introduction of multiple genes into elite rice varieties—evaluation of transgene stability, gene expression, and field performance of herbicide-resistant transgenic plants
Transgenic rice plants from indica, japonica, and javanica varieties that express several foreign genes were generated via particle gun bombardment of immature embryos. Molecular and genetic characterization of large numbers of these plants (more than 500 independent transgenic plants) provided information on structure, expression, and stability of integrated DNA through multiple generations. Such evaluations were carried out in the greenhouse (five generations) and in the field (three generations). Stability of foreign DNA was found to be dependent on the nature of the promoter and the transgene, and in specific cases, on gene copy number. A general conclusion of the results presented here is that direct DNA transfer utilizing electric discharge particle bombardment for the delivery of foreign DNA into rice tissue results in the recovery of large numbers of independently derived transgenic plants in a variety-independent fashion.
Christou, P.; Vain, P.; Kohli, A.; Leech, M.; Oard, J.; Linscombe, S. Introduction of multiple genes into elite rice varieties&#8212;evaluation of transgene stability, gene expression, and field performance of herbicide-resistant transgenic plants. Presented at Rice Genetics III. Proceedings of the Third International Rice Genetics Symposium, Manila, Philippines, 16 &#8211; 20 October 1995. (1996) pp. 223-238