In silico analysis and molecular characterization of rice oxalate oxidase, a candidate gene associated with quantitative resistance to rice blast.

Abstract

Converging evidence points to a role for oxalate oxidases in the defense response of plants to fungal pathogens. Oxalate oxidase is an H<sub>2</sub>O<sub>2</sub> -generating enzyme catalyzing the reaction: oxalate + O<sub>2</sub> + 2H+ - &gt; 2 CO<sub>2</sub> + H<sub>2</sub>O<sub>2</sub>. Active oxygen species, such as H<sub>2</sub>O<sub>2</sub>, have been suggested to be involved in plant defense responses against several fungal pathogens. Oxalate oxidase is a member of the cupin superfamily of functionally diverse proteins with the conserved domains G(x)5HxH(x)3,4E(x)6G and G(x)5PxG(x)2H(x)3N (PFAM00190). Using the candidate gene approach, oxalate oxidase and oxalate oxidase-like proteins have been linked to resistance to rice blast (Magnaporthe grisea). With the complete sequence of the rice genome, along with the genetic and bioinformatic resources for rice, our ability to comprehensively characterize gene families has also been greatly enhanced. These resources include the physical, linkage, and expressed sequence tag (EST) maps, a collection of &gt;130,000 ESTs, gene prediction algorithms, and online resources for comparative grass genomics. Through these resources, we characterized by in silico analysis oxalate oxidase in rice, a candidate gene conferring quantitative resistance to rice blast.

Citation

Carrillo, G.; Reveche, M.; Goodwin, P.; Leach, J.; Leung, H.; Vera Cruz, C.M. In silico analysis and molecular characterization of rice oxalate oxidase, a candidate gene associated with quantitative resistance to rice blast. Presented at the 5th Rice Genetics Symposium, 18-21 November 2005, Manila, Philippines. (2005) 1 pp.

In silico analysis and molecular characterization of rice oxalate oxidase, a candidate gene associated with quantitative resistance to rice blast.

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