An in-house mycobacteriophage amplification assay for detecting rifampin-resistant Mycobacterium tuberculosis showed 100% sensitivity, 97.7% specificity, and 95.2% predictive value for resistance in a test of 129 isolates from a hot spot area of multidrug-resistant M. tuberculosis. The applicability of the test was demonstrated in the routine work flow of a low-resource reference laboratory.
Simboli, N.; Takiff, H.; McNerney, R.; Lopez, B.; Martin, A.; Palomino, J.C.; Barrera, L.; Ritacco, V. In-House Phage Amplification Assay Is a Sound Alternative for Detecting Rifampin-Resistant Mycobacterium tuberculosis in Low-Resource Settings. Antimicrobial Agents and Chemotherapy (2005) 49 (1) 425-427. [DOI: 10.1128/AAC.49.1.425-427.2005]