Enzymes by post—restriction enzyme stability
DNA restriction enzymes, with their widespread applications in many diagnostic procedures (e.g., RFLP, PCR, etc), are the very cornerstone of recombinant DNA technology. Unfortunately, their transport and storage are usually carried out at subzero temperatures, greatly increasing the cost per unit activity and limiting availability in many developing countries.
March, J.B.; Clark, J. Enzymes by post-restriction enzyme stability. Nature Biotechnology (2000) 18 (3) 243. [DOI: 10.1038/73590]