Diversity and genetic differentiation among subpopulations of Gliricidia sepium revealed by PCR-based assays
Randomly amplified polymorphic DNA (RAPD), and a mitochondrial marker based on amplification of the V7 region of the mitochondrial small ribosomal RNA (srRNA) gene, were used to partition genetic variation within a single population of Gliricidia sepium sampled from Guatemala. Seventeen per cent of the variation detected with RAPDs was partitioned among subpopulations and indicated a greater level of discrimination than previously detected with isozymes. Cluster analysis indicated a direct relationship between this variation and the geographical distance between subpopulations. A polymorphism identified within the maternally inherited mitochondrial V7 srRNA product, which relied on digestion with restriction endonucleases, confirmed the genetic subdivision identified with RAPDs, and suggested a relatively limited role for seed in gene dispersal.
Dawson, I.K.; Simons, A.J.; Waugh, R.; Powell, W. Diversity and genetic differentiation among subpopulations of Gliricidia sepium revealed by PCR-based assays. Heredity (1995) 74 (1) 10-18. [DOI: 10.1038/hdy.1995.2]