Diurnal Changes in Transcript and Metabolite Levels during the Iron Deficiency Response of Rice

Rice is highly susceptible to iron deficiency

Abstract

Rice is highly susceptible to iron deficiency due to low secretion levels of the mugineic acid family phytosiderophore (PS) 2′-deoxymugineic acid (DMA) into the rhizosphere. The low levels of DMA secreted by rice have proved challenging to measure and, therefore, the pattern of DMA secretion under iron deficiency has been less extensively studied relative to other graminaceous monocot species that secrete high levels of PS.

Gene expression and metabolite analyses were used to characterise diurnal changes occurring during the iron deficiency response of rice. Iron deficiency inducible genes involved in root DMA biosynthesis and secretion followed a diurnal pattern with peak induction occurring 3–5 h after the onset of light; a result consistent with that of other Strategy II plant species such as barley and wheat.

The results of this study confirmed that rice shares a similar peak time of iron deficiency associated induction of DMA secretion compared to other Strategy II plant species but has less prominent daily fluctuations of DMA secretion. It also revealed metabolic changes associated with the remediation of iron deficiency and mitigation of damage from resulting stress in rice roots.

This work is an output of the HarvestPlus Programme. The Department for International Development is one of the main donors for HarvestPlus.

Citation

Selby-Pham, Jamie, Adrian Lutz, Laura T. Moreno-Moyano, Berin A. Boughton, Ute Roessner, and Alexander A.T. Johnson. 2017. Diurnal changes in transcript and metabolite levels during the iron deficiency response of rice. Rice 10 (14). https://doi.org/10.1186/s12284-017-0152-7.

Diurnal Changes in Transcript and Metabolite Levels during the Iron Deficiency Response of Rice

Published 20 April 2017