Development of non gel-based, high-throughput, and low-cost technologies for marker-assisted selection in rice.
Currently, two obstacles for marker-assisted selection (MAS) are the high cost of genotyping and the limited sample throughput capacity. We are developing new alternative genotyping methods in a collaborative project between IRRI, CIMMYT, and NARES partner institutions. Two methods are being developed as low-cost approaches that can be adopted in laboratories with limited resources. Two other methods are being developed as high-throughput and costeffective methods that could be utilized in core genotyping institutes. We are validating these methods using previously developed markers and characterized or cloned bacterial blight resistance genes (xa5, Xa7, and Xa21) in rice.
Reveche, M.Y.; Mercado, E.; Chen, J.; Collard, B.; Bernardo, M.; Skinner, D.; Wu, J.; Redoña, E.; Kadirvel, P.; Bustamam, M.; Verdier, V.; William, M.; Vera Cruz, C.M. Development of non gel-based, high-throughput, and low-cost technologies for marker-assisted selection in rice. Presented at the 32nd Scientific Meeting of the Crop Science Society of the Philippines (CSSP), 8-12 May 2006 Palawan, Philippines. (2006) 1 pp.