Development of HPLC analytical protocols for quantification of artemisinin in biomass and extracts.
This article is a summary of a report on an MMV-funded project on the subject. Quantification of artemisinin purity and amount in plant material and extracts to date has been characterized by a considerable inconsistency in values. This is likely to be due to the adoption of varied analytical procedures and use of analytical techniques inappropriate to the specific applications. The authors attempt to further develop artemisinin analysis to the point where a universally acceptable reference method is available to the research and end-user communities. Thus, they have developed and validated an HPLC-RI method and optimized an HPLC-ELSD method. They used the gradient HPLC-UV method recommended by the current artemisinin monograph as a comparison for the improved methods presented herein, and show the limitations for its application scope. The data reported should help to allow more reliable laboratory analysis of artemisinin in both pure samples and in Artemisia annua extracts.
Journal of Pharmaceutical and Biomedical Analysis (2009) 49 (4), pp. 908-915 [DOI:10.1016/j.jpba.2009.01.025]