We are developing three alternative genotyping methods for marker-assisted selection (MAS) application in a collaborative project between IRRI, CIMMYT, and NARES partner institutions. Two methods, microarray- and FRET-based genotyping, are being developed as high-throughput and cost-effective techniques that could be utilized in regional laboratory hubs/core genotyping institutes while two other methods, dot blot-based genotyping and PCR-ELISA, are being developed as low-cost approaches that can be adopted in laboratories with limited resources. We are validating these methods using previously developed markers and characterized or cloned bacterial blight resistance genes (xa5, Xa7, and Xa21) in rice.
Mercado, E.; Reveche, M.Y.; Chen, J.; Skinner, D.; Carrillo, G.; Bernardo, M.; William, M.; Xu, Y.; Vera Cruz, C.M.; Collard, B.C.Y. Development of gene-based marker technologies for high throughput genotyping. Presented at the 2006 GCP Annual Research Meeting, Sao Paolo, 12-16 September 2006, Brazil. (2006) 1 pp.