Development and application of an indirect-competitive enzyme-linked immunoassay for aflatoxin M1 in milk and milk-based confectionery

Abstract

High-titer rabbit polyclonal antibodies to aflatoxin M1 (AFM1) were produced by utilizing AFM1-bovine serum albumin (BSA) conjugate as an immunogen. An indirect competitive enzyme-linked immunosorbent assay was standardized for estimating AFM1 in milk and milk products. To avoid the influence of interfering substances present in the milk samples, it was necessary to prepare AFM1 standards in methanol extracts of certified reference material (CRM) not containing detectable AFM1 (93%. The relative cross-reactivity with aflatoxins (AF) and ochratoxin A, assessed as the amount of AFM1 necessary to cause 50% inhibition of binding, was 5% for AFB1 and much less for AFB2, AFG1, and AFG2; there was no reaction with ochratoxin A. AFM1 contamination was measured in retail milk and milk products collected from rural and periurban areas in Andhra Pradesh, India. Of 280 milk samples tested, 146 were found to contain

Citation

Journal of Agricultural and Food Chemistry (2002) 50 (4) 933-937 [DOI: 10.1021/jf011139b]

Development and application of an indirect-competitive enzyme-linked immunoassay for aflatoxin M1 in milk and milk-based confectionery

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