This report describes the isolation and maintenance in culture of Aphanomyces, involved in epizootic ulcerative syndrome (EUS) of fish. Isolates were obtained from Thailand, Bangladesh and the Philippines. Saprophytic, background Aphanomyces isolates were also obtained from Thailand. Isolates of EUS Aphanomyces were compared for their zoosporic capacity in culture, as the ability to produces zoospores is the first step of the pathogenic process. Retention of sporing capacity was demonstrated. Problems in relating free living fungus morphology and size to that during infection within fish were resolved by showing that the C:N ratio controls size of hyphae, and thickness.
Consultancy in tropical mycology. Final Technical Report. 7 pp.