Comparison of the activity of carp and rat β-actin gene regulatory sequences in tilapia and rainbow trout embryos
A comparative study on the level of expression of lacZ reporter constructs driven by equivalent carp and rat β-actin regulatory sequences was carried out in embryos of tilapia and rainbow trout. DNA was microinjected into fertilised tilapia and rainbow trout eggs and the embryos/fry were assayed at various developmental stages for β-galactosidase expression. We provide evidence to demonstrate that the carp β-actin promoter/lacZ reporter gene is expressed at higher levels than the equivalent rat β-actin construct in both species.
Alam, M.S.; Ayad, H.H; Lathe, R.; Lavender, F.L; Lyengar, A.; MacLean, N.; Morley, S.D; Rahman, M.A. Comparison of the activity of carp and rat &#946;-actin gene regulatory sequences in tilapia and rainbow trout embryos. Molecular Reproduction and Development (1996) 45 (2) 117-122. [DOI: 10.1002/(SICI)1098-2795(199610)45:2117::AID-MRD23.0.CO;2-W]