Comparison of latex agglutination, wet preparation, and culture for the detection of Trichomonas vaginalis
Objectives: To compare the performance of three diagnostic methods for Trichomonas vaginalis infection—latex agglutination, saline wet mount, and culture.
Methods: Vaginal swabs from 3807 women attending antenatal clinics were tested for the presence of T vaginalis by latex agglutination. All positives and the following two negatives were tested by wet preparation and culture.
Results: The prevalence of infection by latex agglutination was 5.4%. Using an expanded gold standard based on the wet mount and culture results, the sensitivity of the latex agglutination test was 98.8% (95% CI 95.9 to 99.9) and specificity was 92.1 (89.2 to 94.5). The kappa index for test agreement was 0.93 for latex and culture and 0.88 for latex and wet preparation.
Conclusion: The latex agglutination test is a highly sensitive test for detecting T vaginalis infection. It is a simple rapid test and has the potential for use in screening and diagnostic settings.
Adu-Sarkodie, Y.; Opoku, B.K.; Danso, K.A.; Weiss, H.A.; Mabey, D. Comparison of latex agglutination, wet preparation, and culture for the detection of Trichomonas vaginalis. Sexually Transmitted Infections (2004) 80 (3) 201-203. [DOI: 10.1136/sti.2003.007864]