A co-injection strategy was employed to improve the efficiency of integration of a poorly integrating but commercially important growth hormone gene construct in tilapia. Its co-injection with a reporter gene construct of higher integration efficiency yielded a threefold increase in the integration efficiency of the growth hormone gene construct. In addition, out of 13 transgenic founder tilapia generated, three transmitted the transgenes to G1 and G2 progeny with expected Mendelian inheritance ratios in the G2 generation. We also observed expression of both constructs in a number of founder and G1/G2 individuals. Evidence is provided for the co-ligation of the two constructs and we suggest that this accounts for the increased integration efficiency of growth hormone gene construct and its successful transmission and expression, thus generating lines of novel growth hormone expressing tilapia.
Rahman, M.A.; MacLean N. Co-injection strategy improves integration efficiency of a growth hormone gene construct, resulting in lines of transgenic tilapia (Oreochromis niloticus) expressing an exogenous growth hormone gene. Transgenic Research (1997) 6 (6) 369-378. [DOI: 10.1023/A:1018479232761]