Ten random amplified polymorphic DNA (RAPD) markers specific to chromosome 5Eb of Thinopyrum bessarabicum were detected. Genomic in situ hybridization and standard cytological observations revealed that six of the markers are located on the 5Eb short arm and four are located on the 5Eb long arm. These RAPD markers have been used to confirm the identity of putative 5Eb (5A) and 5Eb (5D) substitution individuals. The potential of RAPDs for the detection of wheat/alien recombinants is discussed.
King, I.P.; Purdie, K.A.; Rezanoor, H.N.; Koebner, R.M.D.; Miller, T.E.; Reader, S.M.; Nicholson, P. Characterization of Thinopyrum bessarabicum chromosome segments in wheat using random amplified polymorphic DNAs (RAPDs) and genomic in situ hybridization. TAG Theoretical and Applied Genetics (1993) 86 (8) 895-900. [DOI: 10.1007/BF00211038]