Application of cryopreservation techniques for the genetic management of commercially important tropical species. Final Project Report.
The prime objective of this study was to develop the technology to establish cryopreserved tilapia sperm banks. This report comprises an executive summary, the objectives of and background to the project, descriptions of the work carried out (procurement and incubation of tilapia gametes, development and improvement of cryopreservation protocols, cryopreservation of tropical fish species, transportation of unfrozen milt and development of a portable field cooler for cryopreservation of tilapia spermatozoa, which was tested during field trips to Sri Lanka, Mexico and the Philippines) and results obtained, and a discussion of the implications of the results. Priority tasks for follow up are discussed. The species used in the study included Puntius conchonius, Orechromis niloticus, Dicentrarchus labrax and Cyprinus carpio. The appendices include a patent application paper for a fixed rate portable cooler, a site-specific cryomanual for the cryounit, ICLARM, and lists of publications and meetings attended.
Application of cryopreservation techniques for the genetic management of commercially important tropical species. Final Project Report, University of Stirling, UK, 71 pp.