An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens

Abstract

A single-step, 5-min lysis method was investigated as a rapid technique to extract genomic DNA from mycobacteria for PCR detection of M. tuberculosis directly from clinical specimens. Of 67 smear-positive clinical specimens, 64 (95.5%) were positive by PCR after this rapid extraction method.

Citation

Journal of Microbiological Methods (2005) 63 (1) 95-98 [doi:10.1016/j.mimet.2005.03.005]

An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens

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