A new cell line was established from the caudal pecundle of a healthy, juvenile warmwater cyprinid, Puntius schwanenfeldi. The cell line has been designated PSP. Cultures grew well in Leibovitz L-15 medium supplemented with 10% fetal calf serum at 25-30ºC and have been passaged more than 60 times since initiation over 2.5 years ago. The PSP line was refractory to infection with a range of viruses associated with outbreaks of the epizootic ulcerative syndrome (EUS), including infectious pancreatic necrosis virus (IPNV) serotype Sp and Ab, and goby birnavirus and snakehead rhabdovirus, but was found to be sensitive to C-type retrovirus released from a persistently infected striped snakehead (Ophicephalus striatus) cell line.
Freichs, G.N.; Karunasager, I.; Millar, S. A New Cell Line from Puntius schwanenfeldi Sensitive to Snakehead Fish Cell Line C-Type Retrovirus. Asian Fisheries Science (1995) 8 (2) 151-158.