A Ds -insertion mutant of OSH6 (Oryza sativa Homeobox 6) exhibits outgrowth of vestigial leaf-like structures, bracts, in rice.

From:
Department for International Development
Published
1 January 2007
Document Type:
Journal Article
Theme:
Agriculture
Authors:
Shoshi Kikuchi, Sung Han Park, Chul Min Kim, Byoung Il Je, Su Hyun Park, Soon Ju Park, Hai Long Piao, Yuan-hu Xuan, Mi Sook Choe, Kouji Satoh, Kon Ho Lee, Young Soon Cha, Byung Ohg Ahn, Hyeon So Ji, Doh Won Yun, Myung Chul Lee, Seok-cheol Suh, Moo Young Eun, Chang-deok Han

Abstract

OSH6 (Oryza sativa Homeobox6) is an ortholog of lg3 (Liguleless3) in maize. We generated a novel allele, termed OSH6-Ds, by inserting a defective Ds element into the third exon of OSH6, which resulted in a truncated OSH6 mRNA. The truncated mRNA was expressed ectopically in leaf tissues and encoded the N-terminal region of OSH6, which includes the KNOX1 and partial KNOX2 subdomains. This recessive mutant showed outgrowth of bracts or produced leaves at the basal node of the panicle. These phenotypes distinguished it from the OSH6 transgene whose ectopic expression led to a \"blade to sheath transformation\" phenotype at the midrib region of leaves, similar to that seen in dominant Lg3 mutants. Expression of a similar truncated OSH6 cDNA from the 35S promoter (35S::ΔOSH6) confirmed that the ectopic expression of this product was responsible for the aberrant bract development. These data suggest that OSH6-Ds interferes with a developmental mechanism involved in bract differentiation, especially at the basal nodes of panicles.

Citation

Planta (2007) 227 (1) 1-12 [doi: 10.1007/s00425-007-0576-1]

A Ds -insertion mutant of OSH6 (Oryza sativa Homeobox 6) exhibits outgrowth of vestigial leaf-like structures, bracts, in rice.

Published 1 January 2007
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