The efficiency of two different immunoaffinity columns and a phenyl bonded-phase column were compared during the extraction, clean-up and quantification of aflatoxin B1 from sorghum and maize. Fluorodensitometry of high performance thin-layer chromatograms was used for quantification of aflatoxin B1. Maize had a simple matrix, and comparable precisions and accuracies were obtained for each of the methods. The sorghum matrix was complex and the bonded-phase procedure was the most accurate and precise method. There was evidence to suggest that the lower aflatoxin B1 recovery from sorghum by immunoaffinity columns is a solvent extraction problem. Better aflatoxin B1 recoveries were obtained from naturally contaminated sorghum and maize when acetonitrile was replaced with acetone as the extraction solvent.
Bradburn, N.; Coker, R.D.; Blunden, G. A comparative study of solvent extraction efficiency and the performance of immunoaffinity and solid phase columns on the determination of aflatoxin B1. Food Chemistry (1995) 52 (2) 179-185. [DOI: 10.1016/0308-8146(94)P4201-P]