Mineral deficiency limits crop production in most soils and in Asia alone, about 50% of rice lands are phosphorus deficient. In an attempt to determine the mechanism of rice adaptation to phosphorus deficiency, changes in proteome patterns associated with phosphorus deficiency have been investigated. We analyzed the parental line Nipponbare in comparison to its near isogenic line (NIL6-4) carrying a major phosphorus uptake QTL (Pup1) on chromosome 12. Using 2-DE, the proteome pattern of roots grown under 1 and 100 M phosphorus were compared. Out of 669 proteins reproducibly detected on root 2-DE gels, 32 proteins showed significant changes in the two genotypes. Of them, 17 proteins showed different responses in two genotypes under stress condition. MS resulted in identification of 26 proteins involved in major phosphorus deficiency adaptation pathways including reactive oxygen scavenging, citric acid cycle, signal transduction, and plant defense responses as well as proteins with unknown function. Our results highlighted a coordinated response in NIL in response to phosphorus deficiency which may confer higher adaptation to nutrient deficiency.
Proteomics (2009) 9 (1) 159-170 [doi: 10.1002/pmic.200800350]