The surface of the southbound carriageway will be replaced with low-noise material, which also reduces spray in wet conditions, between Worcester North at junction 6, and Tewkesbury, Gloucestershire at junction 9.
During the work, the southbound carriageway will be closed in sections, including southbound entry slip roads.
Phase one of the work will be between junction 6 Worcester North and junction 7 at Whittington, from Friday 20 February to Saturday 28 February.
Traffic will be diverted at junction 6 along the A4440, the A44 and re-join the M5 at junction 7.
Phase two of the work will be between junction 7 and junction 8 at Strensham, from Saturday 28 February to Tuesday 24 March.
Traffic will be diverted at junction 7 along the A44, A4440, A38, M50 junction 1, re-joining theM5 at junction 8.
Phase three of the work will be between junction 8 and junction 9 at Tewkesbury, from Tuesday 24 March to Thursday 2 April.
Traffic will be diverted at junction 8 along the M50, A417 and A40, re-joining the M5 at junction 11 for Cheltenham/Gloucester.
All work will be carried out overnight, between 10pm and 6am, Monday to Thursday; 10pm Friday to 8am on Saturday, and 8pm Saturday to 9am on Sunday.
Highways Agency project manager Purnima Kaul said:
The work will lead to an improved, smoother and quieter surface for road users, and reduced traffic noise for those nearby.
To carry it out safely and efficiently we need to close the M5 southbound overnight. Road users should therefore allow extra time for their journeys.
Members of the public should contact the Highways Agency Information Line on 0300 123 5000.
Journalists should contact the Highways Agency press office on 0844 693 1448 and use the menu to speak to the most appropriate press officer.