Functional marker mapping and association analysis of gene W16 in common wheat

Abstract

Objective The objective of this study is to develop and map functional markers for W16, a gene of transcription factor DREB (dehydration responsive element binding) family, to analyze the relationship between haplotypes and phenotypic traits, and provide a basis for the genetic improvement by molecular markers in wheat (Triticum aestivum L.).

Method The W16 was cloned from hexaploid wheat, and its diploid and tetraploid wild relative species. Molecular markers were developed according to sequence polymorphisms. Chromosomal location and genetic mapping of W16 were performed by Chinese Spring nulli-tetrasomic lines and a doubled haploid (DH) population (Hanxuan 10 × Lumai 14). The relevance between W16 haplotypes and phenotypic traits was analyzed by using a natural population consisted of 154 historical wheat accessions.

Result The W16 was mapped in the interval between marker CWM517 and WMC20 on chromosome 1A in Chinese Spring nulli-tetrasomic lines and DH population, and the distances were 7.8 cM and 19.4 cM from the left and right flanking markers. In the natural populations, three haplotypes of W16 were detected which were associated with spike per plant, grain per spike, spikelet per spike and grain plumpness.

Conclusion The present research mapped W16 on the chromosomes 1A. HapⅡ is considered as a potential superior haplotype for the improvement of spike per plant and grain plumpness, while HapⅢ is a potential superior haplotype for grain per spike. These functional markers and association analysis results provide important information for the molecular breeding of wheat.

Citation

Lei MengLin; Li Ang; Chang XiaoPing; Xu ZhaoShi; Ma YouZhi; Liu HuiMin; Jing RuiLian. Functional marker mapping and association analysis of gene W16 in common wheat. Scientia Agricultura Sinica (2012) 45 (9) 1667-1675.

Functional marker mapping and association analysis of gene W16 in common wheat

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