The susceptibility of snakehead rhabdovirus to treatment at 20°C with 5 commercially available disinfectants was examined. No reduction in virus infectivity occurred following exposure to 5 ppm malachite green for 6 hours. Treatment of infective cell culture fluids with 2% formalin resulted in > 99.9% reduction in virus titre within 5 minutes and complete inactivation within 30 minutes, but a negligible loss in infectivity after exposure to 0.025% formalin for 1 hour. Suspensions of virus in distilled water were completely inactivated within 5 minutes by 12.5 ppm chlorine, 50 ppm iodine, or a 1:2000 dilution of a peroxygen disinfectant. In the presence of serum in infective cell culture fluids, however, > 50 ppm chlorine was required to inactivate the agent and no measurable reduction in infectivity was observed following treatment with 500 ppm iodine for 30 minutes.
Frerichs, G.N. Efficacy of chemical disinfectants against snakehead rhabdovirus. Journal of Applied Ichthyology (1990) 6 (2) 117-123. [DOI: 10.1111/j.1439-0426.1990.tb00509.x]