Genomic in situ hybridization (GISH) was used to study the nature of homoeologous chiasma formation in crosses between Triticum durum cv. Creso, homozygous for the ph1c mutation and Thinopyrum bessarabicum. The relative frequencies of wheat/wheat and wheat/Th. bessarabicum chiasma formation were determined. Pairing between apparently non-homologous Th. bessarabicum chromosomes was also observed. The potential of GISH as a tool for analysing homoeologous chiasma formation in wheat/alien hybrids is discussed.
King, I.P.; Purdie, K.A.; Orford, S.E.; Reader, S.M.; Miller, T.E. Detection of homoeologous chiasma formation in Triticum durum × Thinopyrum bessarabicum hybrids using genomic in situ hybridization. Heredity (1993) 71 (4) 369-372. [DOI: 10.1038/hdy.1993.151]